The photo-induced voltage in an individual K enriched MoO(3) nanobundle was studied with localized focused laser beam irradiation. Without an external bias voltage, a significant photo-induced voltage (36.5 mV) was produced in a single nanobundle under low laser power (2.2 mW). Remarkably, the amplitude and polarity of the voltage could be controlled by the location of the focused laser spot. Unlike the common photo-response that comes from metal-semiconductor junction or PN junction in hybrid nanomaterial, the observed photo-induced effect is from the nanobundle itself, attributed to the small band gap of the material.