Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry

Angew Chem Int Ed Engl. 2012 Dec 21;51(52):12992-6. doi: 10.1002/anie.201206745. Epub 2012 Nov 22.

Abstract

Antibody profiling: native mass spectrometry analysis of intact antibodies can be achieved with improved speed, sensitivity, and mass resolution by using a modified orbitrap instrument. Complex mixtures of monoclonal antibodies can be resolved and their glycan "fingerprints" can be profiled. Noncovalent interactions are maintained, thus allowing antibody-antigen binding to be measured.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology
  • Antigens / immunology
  • Glycosylation
  • HEK293 Cells
  • Humans
  • Interleukin-6 / immunology
  • Mass Spectrometry*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Interleukin-6
  • Recombinant Proteins