Quantification of IncP-1 plasmid prevalence in environmental samples

Appl Environ Microbiol. 2013 Feb;79(4):1410-3. doi: 10.1128/AEM.03728-12. Epub 2012 Dec 14.

Abstract

To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacteroidetes / genetics*
  • Biotransformation
  • Environmental Microbiology*
  • Pesticides / metabolism
  • Plasmids / analysis*
  • Plasmids / classification
  • Real-Time Polymerase Chain Reaction

Substances

  • Bacterial Proteins
  • KorB protein, Plasmid RK2
  • Pesticides