Abstract
Due to limited available therapeutic options, developing new lead compounds against hepatitis C virus is an urgent need. Human La protein stimulates hepatitis C virus translation through interaction with the hepatitis C viral RNA. A cyclic peptide mimicking the β-turn of the human La protein that interacts with the viral RNA was synthesized. It inhibits hepatitis C viral RNA translation significantly better than the corresponding linear peptide at longer post-treatment times. The cyclic peptide also inhibited replication as measured by replicon RNA levels using real time RT-PCR. The cyclic peptide emerges as a promising lead compound against hepatitis C.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Antiviral Agents / chemistry
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Antiviral Agents / metabolism
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Antiviral Agents / pharmacology*
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Hepacivirus / drug effects*
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Hepacivirus / genetics
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Hepacivirus / physiology
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Hepatitis C / drug therapy
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Hepatitis C / genetics
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Hepatitis C / metabolism
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Hepatitis C / virology*
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Humans
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Peptides, Cyclic / chemistry
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Peptides, Cyclic / genetics
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Peptides, Cyclic / metabolism
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Peptides, Cyclic / pharmacology*
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Phosphoproteins / chemistry*
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Protein Binding / drug effects
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Protein Biosynthesis / drug effects
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Viral Proteins / genetics
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Viral Proteins / metabolism
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Virus Replication / drug effects
Substances
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Antiviral Agents
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La protein, human
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Peptides, Cyclic
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Phosphoproteins
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Viral Proteins