Abstract
The histone-lysine N-methyltransferase, enhancer of zeste homologue 2 (EZH2), functions as a transcriptional repressor and plays an important role in the development of various types of cancer. In this study, we observed the increased EZH2 expression in the Bel/FU multidrug-resistant hepatocellular carcinoma (HCC) cell line. The RNA interference (RNAi)-mediated depletion of EZH2 expression in the Bel/FU cells led to decreased multidrug resistance protein 1 (MDR1) expression, which resulted in increased apoptosis and sustained G1/S phase arrest. Moreover, siRNA targeting EZH2 sensitized Bel/FU cells to 5-fluorouracil treatment. These findings suggest that EZH2 plays a role in the development of multidrug resistance and may represent a novel therapeutic target for multidrug-resistant HCC.
Publication types
-
Research Support, Non-U.S. Gov't
-
Retracted Publication
MeSH terms
-
ATP Binding Cassette Transporter, Subfamily B
-
ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
-
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
-
Antimetabolites, Antineoplastic / pharmacology*
-
Apoptosis
-
Carcinoma, Hepatocellular
-
Cell Line, Tumor
-
Drug Resistance, Multiple
-
Drug Resistance, Neoplasm*
-
Enhancer of Zeste Homolog 2 Protein
-
Fluorouracil / pharmacology*
-
G1 Phase Cell Cycle Checkpoints
-
Gene Knockdown Techniques
-
Humans
-
Liver Neoplasms
-
Polycomb Repressive Complex 2 / genetics*
-
Polycomb Repressive Complex 2 / metabolism
-
RNA Interference*
-
RNA, Small Interfering / genetics
Substances
-
ABCB1 protein, human
-
ATP Binding Cassette Transporter, Subfamily B
-
ATP Binding Cassette Transporter, Subfamily B, Member 1
-
Antimetabolites, Antineoplastic
-
RNA, Small Interfering
-
EZH2 protein, human
-
Enhancer of Zeste Homolog 2 Protein
-
Polycomb Repressive Complex 2
-
Fluorouracil