Imaging the static dielectric constant in vitro and in living cells by a bioconjugable GFP chromophore analog

Chem Commun (Camb). 2013 Feb 28;49(17):1723-5. doi: 10.1039/c3cc38071h.

Abstract

A fluorescent probe structurally similar to the GFP chromophore is demonstrated to report the local static dielectric constant. This probe can be chemically functionalized for selective targeting at the intracellular level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzoates / chemistry*
  • CHO Cells
  • Cholesterol / chemistry
  • Cricetinae
  • Cricetulus
  • Fluorescent Dyes / chemistry
  • Furans / chemistry*
  • Green Fluorescent Proteins / chemistry*
  • Microscopy, Confocal
  • Static Electricity
  • Unilamellar Liposomes / chemistry

Substances

  • Benzoates
  • Fluorescent Dyes
  • Furans
  • Unilamellar Liposomes
  • methyl 4-((E)-(5-(3,4-dimethoxyphenyl)-2-oxofuran-3(2H)-ylidene)methyl)benzoate
  • Green Fluorescent Proteins
  • Cholesterol