[Effect of ginsenoside Rg1 on functional expression of human neural stem cells: a patch clamp study]

Zhongguo Zhong Yao Za Zhi. 2012 Nov;37(22):3477-80.
[Article in Chinese]

Abstract

Objective: To observe the effects of ginsenoside Rg1 on the functional expression of human neural stem cells (hNSCs).

Method: The membrane electrophysiological properties and sodium and potassium ion channels in the hNSCs induced by Rg1 were analyzed using the whole-cell patch-clamp.

Result: On the 7th day, the neuron-like cells derived from ginsenoside Rg1 (20 mg x L(-1))-induced NSCs show: (1) The resting membrane potential: (-45.70 +/- 2.63) mV, the membrane capacitance: (26.89 +/- 1.91) pF, the membrane input impedance: (877.51 +/- 20.44) MH (P < 0.05 compared with the control group, respectively); (2) The detection rate of inward sodium current which is rapidly activated and inactivated in voltage-dependence was 50%, and its average peak value was (711.48 +/- 158.03) pA (P < 0.05 compared with the control group); (3) The outward potassium currents were composed of rapidly activated and inactivated transient outward potassium current and delayed rectifier outward potassium current, and its average peak value was (1 070.42 +/- 177.18) pA (P < 0.05 compared with the control group).

Conclusion: Ginsenoside Rg1 can promote the functional expression and maturity of hNSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Gene Expression / drug effects
  • Ginsenosides / pharmacology*
  • Humans
  • Membrane Potentials / drug effects
  • Neural Stem Cells / cytology
  • Neural Stem Cells / drug effects*
  • Patch-Clamp Techniques
  • Plant Extracts / pharmacology*
  • Potassium Channels / genetics
  • Potassium Channels / metabolism
  • Sodium Channels / genetics
  • Sodium Channels / metabolism

Substances

  • Ginsenosides
  • Plant Extracts
  • Potassium Channels
  • Sodium Channels
  • ginsenoside Rg1