Physical mapping and cloning of RAD56

Gene. 2013 Apr 25;519(1):182-6. doi: 10.1016/j.gene.2013.01.044. Epub 2013 Feb 9.

Abstract

Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Bleomycin / adverse effects
  • Camptothecin / adverse effects
  • Chromosome Mapping*
  • Cloning, Molecular*
  • DNA Damage
  • DNA Repair
  • DNA, Fungal / genetics
  • Hydroxyurea / adverse effects
  • Methyl Methanesulfonate / adverse effects
  • Mutation*
  • N-Terminal Acetyltransferase B / genetics*
  • N-Terminal Acetyltransferase B / metabolism
  • Phenotype
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Sequence Analysis, DNA
  • X-Rays / adverse effects

Substances

  • DNA, Fungal
  • Nat3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Bleomycin
  • Zeocin
  • Methyl Methanesulfonate
  • N-Terminal Acetyltransferase B
  • Hydroxyurea
  • Camptothecin