Conjugation of a monoclonal antibody with a DTPA modified random copolymer of hydroxyethyl methylacrylate and methyl methacrylate

Int J Rad Appl Instrum B. 1990;17(3):321-9. doi: 10.1016/0883-2897(90)90059-a.

Abstract

A new approach for covalent coupling diethylenetriaminepentaacetic acid (DTPA) molecules to a partially reduced monoclonal antibody utilizes a malemide modified copolymer of hydroxyethyl methylacrylate and methyl methacrylate (DTPA copolymer) prepared by the group transfer polymerization (GTP) method. An average of 6 DTPA molecules were incorporated per mol maleimeide DTPA copolymer and 1.5 mol maleimide DTPA copolymer per mol antibody. Maleimide DTPA copolymer modified antibody was intramolecularly cross-linked, reduced immunoactivity and had a high in vivo liver uptake.

MeSH terms

  • Acrylates / chemical synthesis*
  • Animals
  • Antibodies, Monoclonal*
  • Indium Radioisotopes
  • Isotope Labeling / methods
  • Methacrylates / chemical synthesis*
  • Methacrylates / pharmacokinetics
  • Mice
  • Pentetic Acid / chemical synthesis*
  • Pentetic Acid / pharmacokinetics
  • Tissue Distribution

Substances

  • Acrylates
  • Antibodies, Monoclonal
  • DTPA copolymer
  • Indium Radioisotopes
  • Methacrylates
  • Pentetic Acid