Conversion of high grade lymphoma tumor cell line to intermediate grade with TPA and bryostatin 1 as determined by polypeptide analysis on 2D gel electrophoresis

Hematol Oncol. 1990 Mar-Apr;8(2):81-9. doi: 10.1002/hon.2900080203.

Abstract

A high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used for total cellular polypeptide mapping of two established lymphoma cell lines; MANCA, a Burkitt's line representing a high grade lymphoma (HGL) and WSU-NHL (nodular histiocytic lymphoma) representing an intermediate grade lymphoma (IGL). Gels were digitized and analysed with an image scanning computer using the Elsie 4 system. Polypeptide mapping revealed striking similarities in 1100 polypeptide spots in both types. However, three polypeptides were unique to HGL (Molecular mass/isoelectric point (Mr/PI): 39/4.4, 35/5.6, 33/4.8), and one to IGL (95/4.7). In order to investigate the kinetics of expression of these polypeptides, the two cell lines were treated with two protein kinase C (PKC) activators, tumour promoting 12-O tetradecanoylphorbol 13-acetate (TPA) and bryostatin 1. 2D-PAGE of the treated cells revealed that the HGL line loses its unique polypeptides and expresses a new one. The new polypeptide has the same Mr and PI as that unique to the untreated IGL (95/4.7). TPA or bryostatin 1 treatment of the IGL line for 72 h induced no significant changes. Our data show a unidirectional change from HGL to IGL, supporting the clinical notion that HGL is less differentiated than IGL. It also shows the similarity in the mode of action of bryostatin 1 and TPA in inducing these polypeptide changes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bryostatins
  • Cell Differentiation
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Lactones / pharmacology*
  • Lymphoma / analysis*
  • Lymphoma / genetics
  • Lymphoma / pathology
  • Macrolides
  • Peptides / analysis*
  • Phenotype
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Bryostatins
  • Lactones
  • Macrolides
  • Peptides
  • bryostatin 1
  • Tetradecanoylphorbol Acetate