Objective: To study the effect of different plant growth substance and activated charcoal on rooting in culture seedling of Begonia fimbristpula on Dinghushan mountain.
Methods: Tissue culture single factor experiment method was used.
Results: NAA 0. 3 mg/L + IBA 0. 2 mg/L preferably induction adventitious bud clump with corm to take rooting, but the number of adventitious root were less, short and small, callow shoot more germination. 300 mg/L activated carbon obviously increased radicate quality and inhibited fine buds point differentiation, root number up to 15.5 institia, root length range was 2.0-5.1 cm, root system developed. Tissue culture seedlings were higher, corn and leaf were good quality, strong growth. Took root of seedling cultivation with bulb for bush in the form of scattered bud planted to peat soil: perlite (3:1) mixed in matrix, after the transplant survival rate reached 100%, plant form seedlings fast, grew exuberant.
Conclusion: MS with sucrose 30 g/L + NAA 0.3 mg/L + IBA 0.2 mg/L + activated carbon 300 mg/L + carrageenan 7.0 g/L as the tissue culture seedling of Begonia fimbristipula radicate system, is rapid propagation and preserve local unique plant in an effective way.