Abstract
The Meliaceae family of plants has been shown to contain a vast number of compounds with the potential to be developed for medicinal purposes. We have previously reported the isolation of limonoids from a plant in the Meliaceae family named Chisocheton ceramicus. Ceramicine B was identified as an active compound in inhibiting lipid droplets accumulation (LDA) in the mouse preadipocyte cell line MC3T3-G2/PA6. The presence of ceramicine B was found to inhibit the expression of glucose transporter type 4, lipoprotein lipase, and 11-beta hydroxysteroid dehydrogenase mRNA, and also adipogenic master regulator, peroxisome proliferator-activated receptor-γ, and CCAAT-enhancer-binding protein-α (C/EBPα) mRNA. However, for early adipogenic regulators, such as C/EBPβ and C/EBPδ, and intermediary adipogenic regulators, Krüppel-like factors were unaffected. Western blot analysis showed that ceramicine B was found to inhibit the phosphorylation of Forkhead box O1 (Foxo1), a key process in the insulin signaling pathway. This suggested that the mechanism of anti-LDA activity of ceramicine B was partly via the inhibition of Foxo1 phosphorylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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11-beta-Hydroxysteroid Dehydrogenases / genetics
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11-beta-Hydroxysteroid Dehydrogenases / metabolism
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3T3 Cells
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Adipocytes / drug effects*
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Adipocytes / metabolism
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Adipogenesis / drug effects*
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Animals
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Anti-Obesity Agents / isolation & purification
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Anti-Obesity Agents / pharmacology*
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CCAAT-Enhancer-Binding Proteins / genetics
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CCAAT-Enhancer-Binding Proteins / metabolism
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Dose-Response Relationship, Drug
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Forkhead Box Protein O1
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Forkhead Transcription Factors / metabolism
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Glucose Transporter Type 4 / genetics
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Glucose Transporter Type 4 / metabolism
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Kruppel-Like Transcription Factors / metabolism
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Limonins / isolation & purification
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Limonins / pharmacology*
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Lipoprotein Lipase / genetics
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Lipoprotein Lipase / metabolism
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Meliaceae* / chemistry
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Mice
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PPAR gamma / genetics
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PPAR gamma / metabolism
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Phosphorylation
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Phytotherapy
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plants, Medicinal
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RNA, Messenger / metabolism
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Signal Transduction / drug effects
Substances
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Anti-Obesity Agents
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CCAAT-Enhancer-Binding Proteins
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CEBPA protein, mouse
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Forkhead Box Protein O1
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Forkhead Transcription Factors
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Foxo1 protein, mouse
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Glucose Transporter Type 4
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Kruppel-Like Transcription Factors
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Limonins
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PPAR gamma
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Plant Extracts
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RNA, Messenger
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Slc2a4 protein, mouse
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ceramicine B
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11-beta-Hydroxysteroid Dehydrogenases
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Lipoprotein Lipase