Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus

J Nat Med. 2014 Jan;68(1):22-30. doi: 10.1007/s11418-013-0755-2. Epub 2013 Mar 14.

Abstract

The Meliaceae family of plants has been shown to contain a vast number of compounds with the potential to be developed for medicinal purposes. We have previously reported the isolation of limonoids from a plant in the Meliaceae family named Chisocheton ceramicus. Ceramicine B was identified as an active compound in inhibiting lipid droplets accumulation (LDA) in the mouse preadipocyte cell line MC3T3-G2/PA6. The presence of ceramicine B was found to inhibit the expression of glucose transporter type 4, lipoprotein lipase, and 11-beta hydroxysteroid dehydrogenase mRNA, and also adipogenic master regulator, peroxisome proliferator-activated receptor-γ, and CCAAT-enhancer-binding protein-α (C/EBPα) mRNA. However, for early adipogenic regulators, such as C/EBPβ and C/EBPδ, and intermediary adipogenic regulators, Krüppel-like factors were unaffected. Western blot analysis showed that ceramicine B was found to inhibit the phosphorylation of Forkhead box O1 (Foxo1), a key process in the insulin signaling pathway. This suggested that the mechanism of anti-LDA activity of ceramicine B was partly via the inhibition of Foxo1 phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 11-beta-Hydroxysteroid Dehydrogenases / genetics
  • 11-beta-Hydroxysteroid Dehydrogenases / metabolism
  • 3T3 Cells
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adipogenesis / drug effects*
  • Animals
  • Anti-Obesity Agents / isolation & purification
  • Anti-Obesity Agents / pharmacology*
  • CCAAT-Enhancer-Binding Proteins / genetics
  • CCAAT-Enhancer-Binding Proteins / metabolism
  • Dose-Response Relationship, Drug
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors / metabolism
  • Glucose Transporter Type 4 / genetics
  • Glucose Transporter Type 4 / metabolism
  • Kruppel-Like Transcription Factors / metabolism
  • Limonins / isolation & purification
  • Limonins / pharmacology*
  • Lipoprotein Lipase / genetics
  • Lipoprotein Lipase / metabolism
  • Meliaceae* / chemistry
  • Mice
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Phosphorylation
  • Phytotherapy
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plants, Medicinal
  • RNA, Messenger / metabolism
  • Signal Transduction / drug effects

Substances

  • Anti-Obesity Agents
  • CCAAT-Enhancer-Binding Proteins
  • CEBPA protein, mouse
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors
  • Foxo1 protein, mouse
  • Glucose Transporter Type 4
  • Kruppel-Like Transcription Factors
  • Limonins
  • PPAR gamma
  • Plant Extracts
  • RNA, Messenger
  • Slc2a4 protein, mouse
  • ceramicine B
  • 11-beta-Hydroxysteroid Dehydrogenases
  • Lipoprotein Lipase