Abstract
Transcriptional changes in the nitrogen stress response (NSR) of wild type S. meliloti Rm1021, and isogenic strains missing both PII proteins, GlnB and GlnK, or carrying a ΔglnD-sm2 mutation were analyzed using whole-genome microarrays. This approach allowed us to identify a number of new genes involved in the NSR and showed that the response of these bacteria to nitrogen stress overlaps with other stress responses, including induction of the fixK2 transcriptional activator and genes that are part of the phosphate stress response. Our data also show that GlnD and GlnBK proteins may regulate many genes that are not part of the NSR. Analysis of transcriptome profiles of the Rm1021 ΔglnD-sm2 strain allowed us to identify several genes that appear to be regulated by GlnD without the participation of the PII proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adaptation, Biological*
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Bacterial Proteins / genetics*
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Gene Expression Profiling*
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Gene Expression Regulation, Bacterial
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Models, Biological
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Molecular Sequence Annotation
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Mutation
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Nitrogen / metabolism*
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PII Nitrogen Regulatory Proteins / genetics
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PII Nitrogen Regulatory Proteins / metabolism
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Phosphates / metabolism
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Polysaccharides, Bacterial / biosynthesis
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Reproducibility of Results
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Sinorhizobium meliloti / genetics*
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Sinorhizobium meliloti / metabolism*
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Stress, Physiological*
Substances
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Bacterial Proteins
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PII Nitrogen Regulatory Proteins
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Phosphates
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Polysaccharides, Bacterial
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succinoglycan
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Nitrogen
Grants and funding
This work was supported by the Agricultural Research Center at Washington State University and grant DE-FG03-96ER20225 from the Energy Biosciences Program at the United States Department of Energy. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript