Translating the concept of peptide labeling with 5-deoxy-5-[18F]fluororibose into preclinical practice: 18F-labeling of Siglec-9 peptide for PET imaging of inflammation

Xiang-Guo Li; Anu Autio; Helena Ahtinen; Kerttuli Helariutta; Heidi Liljenbäck; Sirpa Jalkanen; Anne Roivainen; Anu J Airaksinen

doi:10.1039/c3cc40738a

Translating the concept of peptide labeling with 5-deoxy-5-[18F]fluororibose into preclinical practice: 18F-labeling of Siglec-9 peptide for PET imaging of inflammation

Chem Commun (Camb). 2013 May 7;49(35):3682-3684. doi: 10.1039/c3cc40738a. Epub 2013 Mar 27.

Authors

Xiang-Guo Li¹, Anu Autio², Helena Ahtinen², Kerttuli Helariutta³, Heidi Liljenbäck⁴, Sirpa Jalkanen⁵, Anne Roivainen⁴, Anu J Airaksinen³

Affiliations

¹ Department of Pharmacology, Drug Development and Therapeutics, University of Turku, FI-20014 Turku, Finland. xiali@utu.fi and Turku PET Centre, University of Turku and Turku University Hospital, FI-20521 Turku, Finland. anne.roivainen@utu.fi.
² Turku PET Centre, University of Turku and Turku University Hospital, FI-20521 Turku, Finland. anne.roivainen@utu.fi.
³ Laboratory of Radiochemistry, Department of Chemistry, University of Helsinki, FI-00014 Helsinki, Finland. anu.airaksinen@helsinki.fi.
⁴ Turku PET Centre, University of Turku and Turku University Hospital, FI-20521 Turku, Finland. anne.roivainen@utu.fi and Turku Center for Disease Modeling, University of Turku, FI-20014 Turku, Finland.
⁵ MediCity Research Laboratory and Department of Medical Microbiology and Immunology, University of Turku, FI-20014 Turku, Finland.

PMID: 23536117
DOI: 10.1039/c3cc40738a

Abstract

Peptide glycosylation with 5-deoxy-5-[(18)F]fluororibose was translated into preclinical settings. The novel (18)F-labeled Siglec-9 peptide was produced using an automated synthesis procedure. The (18)F-labeled Siglec-9 peptide showed favorable binding in the animal model of inflammation in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amine Oxidase (Copper-Containing) / metabolism
Animals
Antigens, CD / chemistry*
Cell Adhesion Molecules / metabolism
Inflammation / diagnosis
Inflammation / metabolism
Peptides / chemistry*
Positron-Emission Tomography / methods
Rats
Ribose / analogs & derivatives
Ribose / chemistry
Sialic Acid Binding Immunoglobulin-like Lectins / chemistry*

Substances

5-deoxy-5-(18F)fluororibose
Antigens, CD
Cell Adhesion Molecules
Peptides
Sialic Acid Binding Immunoglobulin-like Lectins
Ribose
Amine Oxidase (Copper-Containing)
vascular adhesion protein-1, rat