Abstract
We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers / metabolism
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Chromosome Mapping
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DNA Primers / genetics
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Gene Expression Profiling / methods*
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Genes, Reporter / genetics*
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Genetic Vectors / genetics*
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Gram-Negative Bacteria / genetics*
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Gram-Negative Bacteria / isolation & purification*
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Luciferases, Bacterial / genetics*
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Plasmids / genetics
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Pseudomonas aeruginosa / metabolism
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Trimethoprim Resistance / genetics
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Trimethoprim Resistance / physiology
Substances
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Biomarkers
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DNA Primers
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Luciferases, Bacterial