Overexpressions of microRNA-9 and microRNA-200c in human breast cancers are associated with lymph node metastasis

Cancer Biother Radiopharm. 2013 May;28(4):283-8. doi: 10.1089/cbr.2012.1293. Epub 2013 Apr 25.

Abstract

Purpose: The aim of this study was to investigate the relationship between the expressions of microRNA-9 (miR-9) and microRNA-200c (miR-200c) in human breast cancers and clinicopathological features.

Methods: We investigated the expressions of miR-9 and miR-200c in 68 patients with breast cancers using the quantitative reverse transcription-polymerase chain reaction method, and assessed the E-cadherin status using the immunohistochemistry method.

Results: The relative expression levels of miR-9 and miR-200c in breast cancer patients with lymph node metastasis were higher than that of patients without lymph node metastasis. The expression of miR-9 correlated inversely with E-cadherin expression.

Conclusions: The results showed that higher expressions of miR-9 and miR-200c in human breast cancers were associated with lymph node metastasis. This study indicated that the elevation of miR-9 and miR-200c in human breast cancers can induce an invasive phenotype and may serve as a molecular diagnostic marker for patients with breast cancer.

Keywords: E-cadherin; breast cancer; metastasis; microRNA-200c; microRNA-9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / chemistry
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cadherins / analysis
  • Carcinoma, Ductal, Breast / chemistry
  • Carcinoma, Ductal, Breast / genetics*
  • Carcinoma, Ductal, Breast / secondary*
  • Female
  • Gene Expression
  • Humans
  • Lymphatic Metastasis
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Middle Aged
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism*

Substances

  • Cadherins
  • MIRN200 microRNA, human
  • MIRN92 microRNA, human
  • MicroRNAs
  • RNA, Neoplasm