Linked expressions of nap and nos genes in a Bradyrhizobium japonicum mutant with increased N(2)O reductase activity

Appl Environ Microbiol. 2013 Jul;79(13):4178-80. doi: 10.1128/AEM.00703-13. Epub 2013 Apr 26.

Abstract

To understand the mechanisms underlying the increased N2O reductase activity in the Bradyrhizobium japonicum 5M09 mutant from enrichment culture under N2O respiration, we analyzed the expression of genes encoding denitrification reductases and regulators. Our results suggest a common regulation of nap (encoding periplasmic nitrate reductase) and nos (encoding N2O reductase).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bradyrhizobium / enzymology
  • Bradyrhizobium / genetics*
  • Bradyrhizobium / growth & development
  • Gene Expression Regulation, Enzymologic / physiology*
  • Gene Regulatory Networks / genetics
  • Gene Regulatory Networks / physiology*
  • Nitrate Reductase / metabolism*
  • Oxidoreductases / metabolism*
  • Real-Time Polymerase Chain Reaction
  • beta-Galactosidase

Substances

  • Oxidoreductases
  • nitrous oxide reductase
  • Nitrate Reductase
  • beta-Galactosidase