Lymphoproliferative response to the 30-kDa protein and a crude lysate from Salmonella typhimurium in patients with ankylosing spondylitis

Scand J Rheumatol. 2013;42(3):232-4. doi: 10.3109/03009742.2012.733960.

Abstract

Objectives: To determine the lymphoproliferative response to the 30-kDa protein (p30) of Salmonella typhimurium in patients with ankylosing spondylitis (AS).

Method: Lymphoproliferative response was determined in peripheral blood mononuclear cells (PBMCs) from 30 patients with AS and 40 healthy subjects. Cells were cultured with concanavalin A (Con A), a crude lysate of S. typhimurium (StCL), or p30. Lymphoproliferation was measured by the MTT assay.

Results: Our data show that the mitogenic response to Con A was similar in both groups studied; however, the lymphoproliferative response to StCL and p30 was statistically higher in AS patients than in healthy subjects.

Conclusions: Our data strongly suggest that S. typhimurium, and particularly p30, are associated with AS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bacterial Proteins / immunology*
  • Concanavalin A / immunology
  • Female
  • Humans
  • Immunity, Cellular
  • Lymphocytes / physiology
  • Male
  • Middle Aged
  • Salmonella Infections / complications*
  • Salmonella typhimurium / immunology*
  • Spondylitis, Ankylosing / immunology*
  • Spondylitis, Ankylosing / microbiology*

Substances

  • Bacterial Proteins
  • Concanavalin A