Lack of association of C-Met-N375S sequence variant with lung cancer susceptibility and prognosis

Int J Med Sci. 2013 Jun 14;10(8):988-94. doi: 10.7150/ijms.5944. Print 2013.

Abstract

Background: Previously, we identified a sequence variant (N375S) of c-Met gene, however, its association with lung cancer risk and prognosis remain undefined.

Patients and methods: We investigated the genotype distribution of the c-Met-N375S sequence variant in 206 lung cancer patients and 207 non-cancer controls in the Taiwanese population by DNA sequencing.

Results: Lung cancer patients with variant A/G and G/G genotypes showed 1.08-fold increased cancer risk when compared to patients with the wild-type A/A genotype (95% CI, 0.60-1.91). There were no significant differences in postoperative survival between c-Met-N375S and wild-type patients. In the cell model, the c-Met-N375S cells showed a decrease in cell death upon treatment with MET inhibitor SU11274 compared to wild-type cells.

Conclusion: Our data suggest that the c-Met-N375S sequence variant may not play a significant role in cancer susceptibility and the prognosis of lung cancer patients. The correlation with chemoresponse of c-Met-N375S is worth further investigation in patients receiving MET therapy.

Keywords: N375S; c-Met; cancer risk; lung cancer.; prognosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Case-Control Studies
  • DNA Primers
  • Female
  • Genetic Predisposition to Disease*
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Male
  • Polymerase Chain Reaction
  • Prognosis
  • Proto-Oncogene Proteins c-met / genetics*

Substances

  • DNA Primers
  • Proto-Oncogene Proteins c-met