Multiplex real-time PCR for prompt diagnosis of an outbreak of human parainfluenza 3 virus in children with acute leukemia

Infection. 2013 Dec;41(6):1171-5. doi: 10.1007/s15010-013-0498-8. Epub 2013 Jul 3.

Abstract

Introduction: Human parainfluenza virus type 3 (HPIV-3) causes significant morbimortality in immunocompromised patients. Outbreaks of severe pneumonitis have been previously described in this setting.

Materials and methods: Retrospective observational study in children diagnosed with acute leukemia and a documented HPIV-3 infection in the context of a nosocomial outbreak occurred in a single center.

Result: During summer 2012, an HPIV-3 infection was detected in six hospitalized children with acute leukemia. All the patients had respiratory symptoms and one of them suffered from parotitis.

Conclusion: Early diagnoses using multiplex real-time polymerase chain reaction (PCR) let us control this outbreak. A phylogenetic analysis confirmed person-to-person transmission of a single HPIV-3 variant.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adolescent
  • Child
  • Child, Preschool
  • Cross Infection / complications
  • Cross Infection / diagnosis*
  • Cross Infection / virology
  • Disease Outbreaks*
  • Female
  • Humans
  • Infant
  • Leukemia / virology*
  • Male
  • Parainfluenza Virus 3, Human / classification
  • Parainfluenza Virus 3, Human / genetics
  • Parainfluenza Virus 3, Human / isolation & purification*
  • Phylogeny
  • Real-Time Polymerase Chain Reaction / methods*
  • Respirovirus Infections / diagnosis*
  • Respirovirus Infections / epidemiology
  • Respirovirus Infections / virology
  • Retrospective Studies
  • Spain / epidemiology