Abstract
Nipah virus causes periodic livestock and human disease with high case fatality rate, and consequent major economic, social and psychological impacts. Fruit bats of the genus Pteropus are the natural reservoir. In this study, we used real time PCR to screen the saliva and urine of P. vampyrus from North Sumatera for Nipah virus genome. A conventional reverse transcriptase (RT-PCR) assay was used on provisionally positive samples to corroborate findings. This is the first report of Nipah virus detection in P. vampyrus in Sumatera, Indonesia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Chiroptera / blood
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Chiroptera / virology*
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Genome, Viral / genetics
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Humans
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Indonesia
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Molecular Sequence Data
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Nipah Virus / genetics
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Nipah Virus / isolation & purification*
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Real-Time Polymerase Chain Reaction
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Sequence Analysis
Grants and funding
IS, AR, AA, and MS are core-funded by the Dept, of Veterinary Services, Indonesia. HF is core-funded by the Queensland Centre for Emerging Infectious Diseases, Dept. of Agriculture, Fisheries & Forestry, Queensland, Australia. PD, TT and JB are core-funded by CSIRO Australian Animal health Laboratory. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.