[The analysis of hantavirus S gene in Apodemus agrarius in Changbai area]

Bing Du Xue Bao. 2013 Jun;29(4):382-5.
[Article in Chinese]

Abstract

To gain more insights into epidemiologic characteristics and genotype of hantavirus in Apodemus agrarius in Changbai Area. Complete hantavirus S segment sequences were amplified by RT-PCR and sequenced. The phylogenetic trees were constructed for analysis of genetic characters of hantavirus. A total of 58 Apodemus agrarius were trapped in the epidemic areas, and complete hantavirus S segment sequences were obtained from 4 lung samples of these rodents (6. 90%0). Phylogenetic analysis of the four S segment sequences indicated that all viruses isolated from Apodemu sagrarius were closely related to genotype 6 of Hantaan virus (95. 8%-96. 3%, nucleotide identity; 98. 6%-99. 5%, amino acid identity), all of them had a specific S387 different from other genotypes of Hantaan virus.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Base Sequence
  • China / epidemiology
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Disease Reservoirs / virology*
  • Genotype
  • Hantavirus Infections / epidemiology
  • Hantavirus Infections / veterinary*
  • Hantavirus Infections / virology
  • Lung / virology
  • Murinae / virology*
  • Orthohantavirus / classification
  • Orthohantavirus / genetics*
  • Orthohantavirus / isolation & purification
  • Phylogeny
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rodent Diseases / virology*
  • Sequence Analysis, DNA
  • Viral Proteins / genetics*

Substances

  • DNA, Complementary
  • RNA, Viral
  • Viral Proteins