Deoxyribozyme-loaded nano-graphene oxide for simultaneous sensing and silencing of the hepatitis C virus gene in liver cells

Chem Commun (Camb). 2013 Sep 25;49(74):8241-3. doi: 10.1039/c3cc43368d.

Abstract

The multifunctional DNAzyme (Dz) delivery system is developed based on nano-sized graphene oxide (nGO) for simultaneous detection and knockdown of the target gene. The Dz/nGO complex system allowed convenient monitoring of HCV mRNA in living cells and silencing of the HCV gene expression by Dz-mediated catalytic cleavage concurrently.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / chemistry
  • Antiviral Agents / metabolism
  • Antiviral Agents / pharmacology
  • Biocatalysis
  • DNA, Catalytic / chemistry
  • DNA, Catalytic / metabolism*
  • Gene Expression Regulation, Viral / drug effects
  • Gene Silencing / drug effects
  • Gene Transfer Techniques*
  • Graphite / chemistry
  • Graphite / metabolism*
  • Graphite / pharmacology
  • Hepacivirus / drug effects
  • Hepacivirus / genetics*
  • Humans
  • Liver / metabolism
  • Liver / pathology
  • Liver / virology
  • Microbial Sensitivity Tests
  • Nanostructures / chemistry*
  • Oxides / chemistry
  • Oxides / metabolism*
  • Oxides / pharmacology
  • Particle Size
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • Structure-Activity Relationship
  • Surface Properties

Substances

  • Antiviral Agents
  • DNA, Catalytic
  • Oxides
  • RNA, Messenger
  • Graphite