The purpose of this protocol is to identify 'footprints' of protein on RNA. However, it can also be used to analyze the secondary structure of RNA. This protocol is optimized for large RNA molecules; however, it can be adapted for the study of small RNAs.
Keywords: Dimethyl sulfate (DMS); Primer extension; Quantification and normalization RNA; RNA extraction; RNA structure experimental analysis; Ribonucleoprotein (RNP).
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