In vitro selection of BNA (LNA) aptamers

Artif DNA PNA XNA. 2013 Apr-Jun;4(2):39-48. doi: 10.4161/adna.25786.

Abstract

Recently, we achieved the first in vitro selection of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) aptamers. High-affinity thrombin-binding aptamers (TBAs) were obtained from DNA-based libraries containing 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) in the 5'-primer region, using the method of capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). Furthermore, a similar selection protocol could provide TBAs that contain B/L nucleotides in both primer and random regions. We review technical challenges involved in the generation of various BNA libraries using analogs of B/L nucleoside-5'-triphosphate and polymerase variants and also discuss applications of these libraries to the selection of BNA (LNA) aptamers, as well as future prospects for their therapeutic and diagnostic uses.

Keywords: 2′,4′-BNA/LNA; Bridged (locked) nucleic acid aptamer; Capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX); KODDNA polymerase; Xeno-nucleic acid (XNA).

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / genetics*
  • Base Sequence
  • Genetic Therapy / methods
  • Humans
  • Inverted Repeat Sequences
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics*
  • SELEX Aptamer Technique

Substances

  • Aptamers, Nucleotide
  • Oligonucleotides
  • locked nucleic acid