Abstract
A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.
Keywords:
N-glycans; bioorganic chemistry; click chemistry; glycosyltransferase; sialic acid.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Click Chemistry / methods
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Fibroblasts / chemistry
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Fibroblasts / metabolism
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Humans
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N-Acetylneuraminic Acid
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Polysaccharides / chemistry*
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Polysaccharides / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sialic Acids / chemical synthesis
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Sialic Acids / chemistry
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Sialyltransferases / chemistry*
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Sialyltransferases / genetics
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Sialyltransferases / metabolism
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beta-D-Galactoside alpha 2-6-Sialyltransferase
Substances
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Polysaccharides
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Recombinant Proteins
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Sialic Acids
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Sialyltransferases
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N-Acetylneuraminic Acid
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beta-D-Galactoside alpha 2-6-Sialyltransferase