Abstract
A heteroduplex tracking assay used to genotype Plasmodium vivax merozoite surface protein 1 was adapted to a capillary electrophoresis format, obviating the need for radiolabeled probes and allowing its use in settings where malaria is endemic. This new assay achieved good allelic discrimination and detected high multiplicities of infection in 63 P. vivax infections in Cambodia. More than half of the recurrent parasitemias sampled displayed identical or highly related genotypes compared to the initial genotype, suggesting that they represented relapses.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Cambodia
-
DNA, Protozoan / chemistry
-
DNA, Protozoan / genetics
-
Electrophoresis, Capillary / methods*
-
Genetic Variation*
-
Heteroduplex Analysis / methods*
-
Humans
-
Malaria, Vivax / parasitology*
-
Merozoite Surface Protein 1 / genetics*
-
Molecular Sequence Data
-
Plasmodium vivax / classification*
-
Plasmodium vivax / genetics*
-
Plasmodium vivax / isolation & purification
-
Recurrence
-
Sequence Analysis, DNA
Substances
-
DNA, Protozoan
-
Merozoite Surface Protein 1