SUMOylation in Trypanosoma brucei

Cornelia Andrea Klein; Dorothea Droll; Christine Clayton

doi:10.7717/peerj.180

SUMOylation in Trypanosoma brucei

PeerJ. 2013 Oct 8:1:e180. doi: 10.7717/peerj.180. eCollection 2013.

Authors

Cornelia Andrea Klein¹, Dorothea Droll, Christine Clayton

Affiliation

¹ Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance , Heidelberg , Germany.

Abstract

Small ubiquitin like modifier (SUMO) proteins are involved in many processes in eukaryotes. We here show that Trypanosoma brucei SUMO (Tb927.5.3210) modifies many proteins. The levels of SUMOylation were unaffected by temperature changes but were increased by severe oxidative stress. We obtained evidence that trypanosome homologues of the SUMO conjugating enzyme Ubc9 (Tb927.2.2460) and the SUMO-specific protease SENP (Tb927.9.2220) are involved in SUMOylation and SUMO removal, respectively.

Keywords: Heat shock; Oxidative stress; SUMO; Small ubiquitin-like modifier; Trypanosoma brucei.

Grants and funding

DD was supported by Sonderforschungsbereich 544 of the Deutsche Forschungsgemeinschaft. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.