Lessons learned from the transformation of natural product discovery to a genome-driven endeavor

J Ind Microbiol Biotechnol. 2014 Feb;41(2):315-31. doi: 10.1007/s10295-013-1361-8. Epub 2013 Oct 19.

Abstract

Natural product discovery is currently undergoing a transformation from a phenotype-driven field to a genotype-driven one. The increasing availability of genome sequences, coupled with improved techniques for identifying biosynthetic gene clusters, has revealed that secondary metabolomes are strikingly vaster than previously thought. New approaches to correlate biosynthetic gene clusters with the compounds they produce have facilitated the production and isolation of a rapidly growing collection of what we refer to as "reverse-discovered" natural products, in analogy to reverse genetics. In this review, we present an extensive list of reverse-discovered natural products and discuss seven important lessons for natural product discovery by genome-guided methods: structure prediction, accurate annotation, continued study of model organisms, avoiding genome-size bias, genetic manipulation, heterologous expression, and potential engineering of natural product analogs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacteria / genetics
  • Bacteria / metabolism
  • Biological Products / chemistry
  • Biological Products / metabolism*
  • Biosynthetic Pathways / genetics
  • Fungi / genetics
  • Fungi / metabolism
  • Genome, Microbial*
  • Genomics / methods

Substances

  • Biological Products