Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture

Methods Mol Biol. 2014:1098:47-55. doi: 10.1007/978-1-62703-718-1_4.

Abstract

Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques / methods*
  • Copepoda / enzymology*
  • Copepoda / genetics
  • Culture Media
  • HEK293 Cells
  • Humans
  • Luciferases / genetics
  • Luciferases / isolation & purification
  • Luciferases / metabolism*
  • Luminescent Measurements / methods*
  • Mycoplasma / isolation & purification*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism

Substances

  • Culture Media
  • Recombinant Proteins
  • Luciferases