Cationically rendered biopolymer surfaces for high protein affinity support matrices

Chem Commun (Camb). 2013 Dec 21;49(98):11530-2. doi: 10.1039/c3cc46414h.

Abstract

The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Biopolymers / chemistry*
  • Cellulose / chemistry*
  • Chitosan / chemistry*
  • Fluorescein-5-isothiocyanate / analogs & derivatives*
  • Fluorescein-5-isothiocyanate / chemistry
  • Rhodamines / chemistry
  • Serum Albumin, Bovine / chemistry*

Substances

  • Biopolymers
  • N-trimethyl chitosan chloride
  • Rhodamines
  • fluorescein isothiocyanate bovine serum albumin
  • Serum Albumin, Bovine
  • tetramethylrhodamine isothiocyanate
  • Cellulose
  • Chitosan
  • Fluorescein-5-isothiocyanate