Genotyping of hepatitis C virus has an important prognostic value for response to antiviral therapy. The results of hepatits C virus genotyping performed between 1994 and 2012 from 1,766 patients of different regions of Chile are reported. Global genotype (Gt) distribution was as follows: 7.87% Gt1a, 72.71% Gt1b, 1.98% Gt2, 16.53% Gt3a, 0.57% Gt4, 0.28% Gt5a and 0.06% Gt6. In most regions the genotype distribution was similar to the global. However, there were some differences, in particular in the south of our country, where 3a is present in more than 30% in some regions.