Detection of chondroitin sulfate proteoglycan 4 (CSPG4) in melanoma

Methods Mol Biol. 2014:1102:523-35. doi: 10.1007/978-1-62703-727-3_28.

Abstract

The tumor antigen chondroitin sulfate proteoglycan 4 (CSPG4) appears to be a useful biomarker to identify melanoma cells and an attractive target to apply antibody-based immunotherapy for the treatment of melanoma. Here we described the reverse transcription-polymerase chain reaction (RT-PCR) method and the immunohistochemical (IHC) staining method to detect the expression of CSPG4 in melanoma cells and tissues.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / genetics
  • Antigens / metabolism*
  • Humans
  • Immunohistochemistry
  • Melanoma / metabolism*
  • Melanoma / pathology
  • Proteoglycans / genetics
  • Proteoglycans / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / pathology

Substances

  • Antigens
  • Proteoglycans
  • chondroitin sulfate proteoglycan 4