Abstract
The hepatocyte growth factor receptor (MET) is a receptor tyrosine kinase (RTK) that has emerged as an important cancer target. Consequently, a number of different inhibitors varying in specificity are currently in clinical development. However, to date, it has been difficult to visualize MET expression, intracellular drug distribution and small molecule MET inhibition. Using a bioorthogonal approach, we have developed two companion imaging drugs based on both mono- and polypharmacological MET inhibitors. We show exquisite drug and target co-localization that can be visualized at single-cell resolution. The developed agents may be useful chemical biology tools to investigate single-cell pharmacokinetics and pharmacodynamics of MET inhibitors.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Biological Transport
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Cell Line, Tumor
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Drug Design
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Humans
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Inhibitory Concentration 50
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Intracellular Space
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Microscopy, Fluorescence / methods
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Models, Molecular
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Molecular Imaging* / methods
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Molecular Structure
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Protein Binding
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Protein Kinase Inhibitors / chemistry
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Protein Kinase Inhibitors / metabolism*
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Protein Kinase Inhibitors / pharmacology
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Proto-Oncogene Proteins c-met / antagonists & inhibitors
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Proto-Oncogene Proteins c-met / chemistry
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Proto-Oncogene Proteins c-met / metabolism*
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Single-Cell Analysis* / methods
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Small Molecule Libraries / chemical synthesis
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Small Molecule Libraries / pharmacology
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Time Factors
Substances
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Protein Kinase Inhibitors
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Small Molecule Libraries
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Proto-Oncogene Proteins c-met