Abstract
The emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. This report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid detection of carbapenemase activity in enterobacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. In a panel of 78 isolates, including 41 carbapenemase-producing strains, the ULPC-MS/MS assay showed 100% agreement with molecular characterization, whereas six carbapenemase-producing isolates were not detected by the modified Hodge test.
MeSH terms
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Acinetobacter baumannii / drug effects
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Acinetobacter baumannii / enzymology
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Acinetobacter baumannii / genetics
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Acinetobacter baumannii / isolation & purification*
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Anti-Bacterial Agents / metabolism*
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / classification
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Chromatography, High Pressure Liquid / methods
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Enzyme Assays
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Gene Expression
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Gram-Negative Bacterial Infections / drug therapy
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Gram-Negative Bacterial Infections / microbiology*
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Humans
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Microbial Sensitivity Tests
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Plasmids
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / enzymology
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / isolation & purification*
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Tandem Mass Spectrometry
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beta-Lactam Resistance / genetics
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beta-Lactamases / classification
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
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beta-Lactams / metabolism*
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beta-Lactams / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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beta-Lactams
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beta-Lactamases
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carbapenemase