Abstract
Bsp-1 is an IgM murine monoclonal antibody raised against the human erythroblastic leukemia cell line (HEL) that reacts with basophils but not neutrophils or eosinophils. Western blotting techniques showed that Bsp-1 reacts with a 45-kilodalton surface antigen on HEL cells. The distribution of Bsp-1 antigen on leukemic cells is confined to a basophilic leukemia cell line, KU812, chronic myeloid leukemia with basophilia, and some cases of acute undifferentiated leukemia. Bsp-1 might therefore be a useful reagent for the study of basophil function and differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal* / isolation & purification
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Antibody Specificity
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Antigen-Antibody Reactions
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Antigens, Surface / analysis*
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Basophils / cytology
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Basophils / immunology*
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Basophils / physiology
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Cell Differentiation
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Cell Line
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Collodion
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Electrophoresis, Polyacrylamide Gel
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Epitopes / analysis
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Fluorescent Antibody Technique
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Hematopoietic Stem Cells / cytology
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Histocytochemistry
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Humans
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Leukemia, Erythroblastic, Acute / pathology
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Leukemia, Myeloid / pathology
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Phenotype
Substances
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Antibodies, Monoclonal
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Antigens, Surface
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Epitopes
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Collodion