Evaluation of pigtail macaques as a model for the effects of copper intrauterine devices on HIV infection

J Med Primatol. 2014 Oct;43(5):349-59. doi: 10.1111/jmp.12096. Epub 2013 Dec 27.

Abstract

Background: Long-acting, hormonal contraception may increase HIV risk. Copper intrauterine devices (IUDs) could serve as non-hormonal alternatives. We pilot a pigtail macaque model for evaluating HIV susceptibility factors during copper IUD use.

Methods: Frameless and flexible GyneFix(®) copper IUDs were surgically implanted into three SHIVSF 162p3 -positive macaques via hysterotomy and monitored for up to 4 months. Four macaques served as non-IUD controls.

Results: All animals retained the devices without complications. No consistent change in vaginal viral RNA or inflammatory cytokines was seen. Two animals had altered menstrual cycles and experienced marked thinning of vaginal epithelium after IUD insertion. Histological examination of uterine tissue at necropsy revealed endometrial ulceration and lymphocytic inflammation with glandular loss at sites of direct IUD contact.

Conclusions: Although the need for insertion surgery could limit its usefulness, this model will allow studies on copper IUDs and SHIV shedding, disease progression, and HIV susceptibility factors.

Keywords: Simian-Human Immunodeficiency Virus (SHIV); contraception; non-human primate; susceptibility; transmission risk.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Contraception
  • Disease Models, Animal
  • Disease Susceptibility / immunology
  • Disease Susceptibility / physiopathology
  • Disease Susceptibility / virology
  • Female
  • HIV / physiology
  • HIV Infections / immunology
  • HIV Infections / physiopathology
  • HIV Infections / prevention & control*
  • HIV Infections / virology
  • Humans
  • Intrauterine Devices, Copper / adverse effects*
  • Macaca nemestrina* / immunology
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / physiopathology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control*
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / isolation & purification
  • Uterus / immunology
  • Virus Shedding