Prereplication-complex formation: a molecular double take?

Nat Struct Mol Biol. 2014 Jan;21(1):20-5. doi: 10.1038/nsmb.2738.

Abstract

In G1, two copies of the MCM2-7 helicase are recruited to each origin of replication. Whereas recruitment of the first MCM2-7 is likely to be analogous to the loading of sliding clamps around DNA, how the second MCM2-7 complex is recruited is highly contentious. Here, we argue that MCM2-7 loading involves specific modifications to the clamp-loading reaction and propose that the first and second MCM2-7 molecules are loaded via similar mechanisms.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA / chemistry*
  • Minichromosome Maintenance Proteins / metabolism
  • Origin Recognition Complex*

Substances

  • Origin Recognition Complex
  • DNA
  • Minichromosome Maintenance Proteins