Inhibition of natural killer-cell mediated cytolysis with monoclonal antibodies to restricted and non-restricted epitopes of the leucocyte common antigen

Immunology. 1987 Jul;61(3):351-6.

Abstract

Three monoclonal antibodies recognizing different epitopes of the leucocyte common molecule, CMRF-11 (against the restricted or B-220 leucocyte common molecule), CMRF-12 and CMRF-26 [each against a different epitope on the non-restricted or T200 leucocyte common (CD45) molecule], were tested for their effects on lymphocyte cytotoxicity. The individual monoclonal antibodies inhibited human natural killer cell-mediated cytolysis (NK-CMC) weakly, but a mixture of CMRF-11 + 12 + 26 antibodies inhibited cytolysis more consistently and to a greater extent. This mixture did not inhibit cytotoxic T lymphocytes derived from secondary mixed lymphocyte cultures. The CMRF-11 + 12 + 26 mix was shown to inhibit a post-conjugate formation stage of lysis at the effector cell level. Inhibition of NK-CMC of a wide range of target cells, including the T-cell lines Jurkat, HSB2 and Molt 4, was demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Cytotoxicity, Immunologic*
  • Epitopes / immunology*
  • Histocompatibility Antigens / immunology*
  • Humans
  • Killer Cells, Natural / immunology*
  • Leukocyte Common Antigens
  • Lymphocyte Culture Test, Mixed
  • T-Lymphocytes, Cytotoxic / immunology

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Histocompatibility Antigens
  • Leukocyte Common Antigens