Fructose-bisphosphate aldolase a is a potential metastasis-associated marker of lung squamous cell carcinoma and promotes lung cell tumorigenesis and migration

Sha Du; Zhuzhu Guan; Lihong Hao; Yang Song; Lan Wang; Linlin Gong; Lu Liu; Xiaoyu Qi; Zhaoyuan Hou; Shujuan Shao

doi:10.1371/journal.pone.0085804

Fructose-bisphosphate aldolase a is a potential metastasis-associated marker of lung squamous cell carcinoma and promotes lung cell tumorigenesis and migration

PLoS One. 2014 Jan 23;9(1):e85804. doi: 10.1371/journal.pone.0085804. eCollection 2014.

Authors

Sha Du¹, Zhuzhu Guan², Lihong Hao³, Yang Song³, Lan Wang³, Linlin Gong³, Lu Liu³, Xiaoyu Qi³, Zhaoyuan Hou⁴, Shujuan Shao²

Affiliations

¹ Department of Histology and Embryology, Dalian Medical University, Liaoning, China ; Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Liaoning, China.
² Department of Histology and Embryology, Dalian Medical University, Liaoning, China ; Liaoning Key Laboratory of Proteomics, Dalian Medical University, Liaoning, China.
³ Department of Histology and Embryology, Dalian Medical University, Liaoning, China.
⁴ Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Liaoning, China.

Abstract

Fructose-bisphosphate aldolase A (ALDOA) is a key enzyme in glycolysis and is responsible for catalyzing the reversible conversion of fructose-1,6-bisphosphate to glyceraldehydes-3-phosphate and dihydroxyacetone phosphate. ALDOA contributes to various cellular functions such as muscle maintenance, regulation of cell shape and mobility, striated muscle contraction, actin filament organization and ATP biosynthetic process. Here, we reported that ALDOA is a highly expressed in lung squamous cell carcinoma (LSCC) and its expression level is correlated with LSCC metastasis, grades, differentiation status and poor prognosis. Depletion of ALDOA expression in the lung squamous carcinoma NCI-H520 cells reduces the capabilities of cell motility and tumorigenesis. These data suggest that ALDOA could be a potential marker for LSCC metastasis and a therapeutic target for drug development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Amino Acid Sequence
Animals
Biomarkers, Tumor / metabolism*
Carcinoma, Squamous Cell / enzymology*
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / secondary
Cell Line, Tumor
Cytoplasm / enzymology
Female
Fructose-Bisphosphate Aldolase / metabolism*
Humans
Kaplan-Meier Estimate
Lung Neoplasms / enzymology*
Lung Neoplasms / mortality
Lung Neoplasms / pathology
Male
Mice
Mice, Nude
Middle Aged
Molecular Sequence Data
Neoplasm Grading
Neoplasm Transplantation

Substances

Biomarkers, Tumor
Fructose-Bisphosphate Aldolase

Grants and funding

This study was supported by grants from National Basic Research Program of China (973 Program, No. 2012CB967003 to S. Shao), Natural Science Foundation of China (NO. 20935004, 81071784 to S. Shao, and 81172028 to Z. Hou). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.