Decomposition of size-exclusion chromatography elution curves of complex branched polymers

Miloš Netopilík; Miroslav Janata

doi:10.1016/j.chroma.2014.01.005

Decomposition of size-exclusion chromatography elution curves of complex branched polymers

J Chromatogr A. 2014 Feb 21:1330:14-9. doi: 10.1016/j.chroma.2014.01.005. Epub 2014 Jan 13.

Authors

Miloš Netopilík¹, Miroslav Janata²

Affiliations

¹ Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Square 2, 162 06 Prague 6, Czech Republic. Electronic address: netopilik@imc.cas.cz.
² Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Square 2, 162 06 Prague 6, Czech Republic.

PMID: 24468240
DOI: 10.1016/j.chroma.2014.01.005

Abstract

A new method for the decomposition of non-baseline-resolved multimodal elution curves of SEC with the concentration, light scattering and viscosity detection is presented. The method makes possible the characterization of the polymer-sample components, represented by the peaks forming multimodal elution curves, individually and reduces also the error in the calculation of molecular-weight averages. The procedure is demonstrated on narrow molecular-weight distribution polystyrene standards and their mixture as well as on a grafted polymer sample.

Keywords: Curve decomposition; Size-exclusion chromatography; Triple detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Gel / methods*
Chromatography, Gel / standards
Light
Molecular Weight
Polystyrenes / analysis*
Reference Standards
Scattering, Radiation
Viscosity

Substances

Polystyrenes