MiR-22 suppresses the proliferation and invasion of gastric cancer cells by inhibiting CD151

Biochem Biophys Res Commun. 2014 Feb 28;445(1):175-9. doi: 10.1016/j.bbrc.2014.01.160. Epub 2014 Feb 1.

Abstract

Gastric cancer (GC) is the second common cause of cancer-related death worldwide. microRNAs (miRNAs) play important roles in the carcinogenesis of GC. Here, we found that miR-22 was significantly decreased in GC tissue samples and cell lines. Ectopic overexpression of miR-22 remarkably suppressed cell proliferation and colony formation of GC cells. Moreover, overexpression of miR-22 significantly suppressed migration and invasion of GC cells. CD151 was found to be a target of miR-22. Furthermore, overexpression of CD151 significantly attenuated the tumor suppressive effect of miR-22. Taken together, miR-22 might suppress GC cells growth and motility partially by inhibiting CD151.

Keywords: CD151; Gastric cancer; Invasion; Migration; Proliferation; miR-22.

MeSH terms

  • 3' Untranslated Regions / genetics
  • Base Sequence
  • Blotting, Western
  • Cell Line
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Cell Proliferation*
  • Cell Survival / genetics
  • Gene Expression Regulation, Neoplastic
  • HEK293 Cells
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Mutation
  • Neoplasm Invasiveness
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology
  • Tetraspanin 24 / genetics*
  • Tetraspanin 24 / metabolism
  • Transfection

Substances

  • 3' Untranslated Regions
  • CD151 protein, human
  • MIRN22 microRNA, human
  • MicroRNAs
  • Tetraspanin 24