Enzymatic mineralization of silk scaffolds

Macromol Biosci. 2014 Jul;14(7):991-1003. doi: 10.1002/mabi.201300513. Epub 2014 Mar 7.

Abstract

The present study focuses on the alkaline phosphatase (ALP) mediated formation of apatitic minerals on porous silk fibroin protein (SFP) scaffolds. Porous SFP scaffolds impregnated with different concentrations of ALP are homogeneously mineralized under physiological conditions. The mineral structure is apatite while the structures differ as a function of the ALP concentration. Cellular adhesion, proliferation, and colonization of osteogenic MC3T3 cells improve on the mineralized SFP scaffolds. These findings suggest a simple process to generate mineralized scaffolds that can be used to enhanced bone tissue engineering-related utility.

Keywords: alkaline phosphatase; mineralization; osteogenesis; scaffolds; silk.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism*
  • Animals
  • Bombyx
  • Calcium Phosphates / metabolism
  • Cattle
  • Cell Survival / drug effects
  • Fibroins / chemistry
  • Fibroins / pharmacology
  • Fibroins / ultrastructure
  • Mice
  • Microscopy, Electron, Scanning
  • Minerals / metabolism*
  • Molecular Weight
  • Osteoblasts / cytology
  • Osteoblasts / drug effects
  • Silk / metabolism*
  • Silk / pharmacology
  • Silk / ultrastructure
  • Spectrometry, X-Ray Emission
  • Spectroscopy, Fourier Transform Infrared
  • Spectrum Analysis, Raman
  • Thermogravimetry
  • Tissue Scaffolds / chemistry*
  • X-Ray Diffraction

Substances

  • Calcium Phosphates
  • Minerals
  • Silk
  • Fibroins
  • calcium phosphate
  • Alkaline Phosphatase