Quantification of deaminase activity-dependent and -independent restriction of HIV-1 replication mediated by APOBEC3F and APOBEC3G through experimental-mathematical investigation

J Virol. 2014 May;88(10):5881-7. doi: 10.1128/JVI.00062-14. Epub 2014 Mar 12.

Abstract

APOBEC3F and APOBEC3G cytidine deaminases potently inhibit human immunodeficiency virus type 1 (HIV-1) replication by enzymatically inserting G-to-A mutations in viral DNA and/or impairing viral reverse transcription independently of their deaminase activity. Through experimental and mathematical investigation, here we quantitatively demonstrate that 99.3% of the antiviral effect of APOBEC3G is dependent on its deaminase activity, whereas 30.2% of the antiviral effect of APOBEC3F is attributed to deaminase-independent ability. This is the first report quantitatively elucidating how APOBEC3F and APOBEC3G differ in their anti-HIV-1 modes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • APOBEC-3G Deaminase
  • Cell Line
  • Cytidine Deaminase / metabolism*
  • Cytosine Deaminase / metabolism*
  • HIV-1 / immunology*
  • HIV-1 / physiology*
  • Host-Pathogen Interactions*
  • Humans
  • Models, Theoretical
  • Virus Replication*

Substances

  • APOBEC3F protein, human
  • Cytosine Deaminase
  • APOBEC-3G Deaminase
  • APOBEC3G protein, human
  • Cytidine Deaminase