A novel aggregation-induced emission fluorescent probe for nucleic acid detection and its applications in cell imaging

Bioorg Med Chem Lett. 2014 Apr 1;24(7):1654-6. doi: 10.1016/j.bmcl.2014.02.071. Epub 2014 Mar 5.

Abstract

A new kind of aggregation-induced emission compound was synthesized and used as the probe of nucleic acid. The characterization of this compound was studied. Both the RNA and DNA were detected by using this probe. And the detection scope of DNA and RNA was different. We researched the selectivity of our probe in double and single strand DNA sequences. The visualization of gel electrophoresis and the cell nucleus imaging were researched as well. Compared with the traditional nucleus dye Hoechst 33258, our probe also has the potential to be nucleus dye. And the cell toxicity was well performed by MTT assays.

Keywords: Aggregation-induced emission; Cell imaging; Dye; Fluorescent; Gel electrophoresis; Nucleic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus* / drug effects
  • DNA / analysis*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / pharmacology
  • HeLa Cells
  • Humans
  • Molecular Imaging / methods*
  • Molecular Structure
  • RNA / analysis*
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • RNA
  • DNA