The 34 kilodalton insulin-like growth factor binding proteins in human cerebrospinal fluid and the A673 rhabdomyosarcoma cell line are human homologues of the rat BRL-3A binding protein

Biochem Biophys Res Commun. 1989 Sep 15;163(2):875-81. doi: 10.1016/0006-291x(89)92303-6.

Abstract

Three members of a family of insulin-like growth factor binding proteins have been identified by nucleotide sequencing of cDNA clones: the binding subunit of the 150 kDa IGF-binding protein complex in human serum, the 30 kDa IGF binding protein in human amniotic fluid, and a 30 kDa binding protein (BP-3A) isolated from the rat BRL-3A cell line. The present study demonstrates by molecular hybridization and immunoreactivity that the human counterpart of rat BP-3A is a 34 kDa IGF binding protein that is present in human cerebrospinal fluid and is synthesized and secreted by the A673 human rhabdomyosarcoma cell line.

Publication types

  • Comparative Study

MeSH terms

  • Amniotic Fluid / metabolism
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Carrier Proteins / metabolism*
  • Cell Line
  • DNA / genetics
  • DNA Probes
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation
  • Humans
  • Insulin-Like Growth Factor Binding Proteins
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Rats
  • Rhabdomyosarcoma / metabolism*
  • Sequence Homology, Nucleic Acid
  • Somatomedins / cerebrospinal fluid*
  • Tumor Cells, Cultured / metabolism

Substances

  • Carrier Proteins
  • DNA Probes
  • Insulin-Like Growth Factor Binding Proteins
  • Somatomedins
  • DNA