Evaluation of the Bio-Rad Geenius HIV 1/2 assay as an alternative to the INNO-LIA HIV 1/2 assay for confirmation of HIV infection

Orna Mor; Fernando Mileguir; Michal Michaeli; Itzchak Levy; Ella Mendelson

doi:10.1128/JCM.01184-14

Evaluation of the Bio-Rad Geenius HIV 1/2 assay as an alternative to the INNO-LIA HIV 1/2 assay for confirmation of HIV infection

J Clin Microbiol. 2014 Jul;52(7):2677-9. doi: 10.1128/JCM.01184-14. Epub 2014 Apr 30.

Authors

Orna Mor¹, Fernando Mileguir², Michal Michaeli², Itzchak Levy³, Ella Mendelson⁴

Affiliations

¹ National HIV Reference Laboratory, Central Virology Laboratory, Ministry of Health, Tel Hashomer, Ramat Gan, Israel orna.mor@sheba.health.gov.il.
² National HIV Reference Laboratory, Central Virology Laboratory, Ministry of Health, Tel Hashomer, Ramat Gan, Israel.
³ Infectious Disease Unit, Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel.
⁴ National HIV Reference Laboratory, Central Virology Laboratory, Ministry of Health, Tel Hashomer, Ramat Gan, Israel Tel Aviv University, Ramat Aviv, Israel.

Abstract

The Bio-Rad Geenius HIV 1/2 assay was evaluated as an alternative to the INNO-LIA HIV 1/2 assay for the confirmation of HIV infection in 198 serum samples reactive to 4th-generation HIV enzyme immunoassays (EIAs). The Geenius assay correctly identified 85% of the samples, compared to 75% identified by the INNO-LIA assay, reduced the number of indeterminate results, and shortened the overall turnaround time.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Clinical Laboratory Techniques / methods*
HIV Antibodies / blood*
HIV Infections / diagnosis*
HIV Infections / virology
HIV-1 / immunology*
HIV-2 / immunology*
Humans
Sensitivity and Specificity

Substances

HIV Antibodies