Ribozyme, antisense RNA, and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro

Mol Cell Biol. 1989 Oct;9(10):4479-87. doi: 10.1128/mcb.9.10.4479-4487.1989.

Abstract

A comparative analysis of ribozyme, antisense RNA, and antisense DNA inhibitors of the in vitro small nuclear ribonucleoprotein U7-dependent histone pre-mRNA processing reaction was performed. RNA molecules complementary to the U7 sequence inhibited in vitro processing of histone pre-mRNA at a sixfold excess over U7. Single-stranded DNA complementary to the entire U7 sequence inhibited the reaction at a 60-fold excess over U7, while a short, 18-nucleotide DNA molecule complementary to the 5' end of U7 inhibited the processing reaction at a 600-fold excess. A targeted ribozyme was capable of specifically cleaving the U7 small nuclear ribonucleoprotein in a nuclear extract and inhibited the U7-dependent processing reaction, but in our in vitro system it required a 1,000-fold excess over U7 for complete inhibition of processing.

MeSH terms

  • Animals
  • Cell-Free System
  • DNA / pharmacology
  • DNA, Antisense
  • Histones / genetics
  • Mice
  • RNA / pharmacology
  • RNA Precursors / metabolism*
  • RNA Processing, Post-Transcriptional / drug effects
  • RNA Processing, Post-Transcriptional / physiology*
  • RNA, Antisense
  • RNA, Catalytic
  • RNA, Ribosomal / genetics
  • RNA, Ribosomal / physiology*
  • Ribonucleoproteins / metabolism*
  • Ribonucleoproteins, Small Nuclear

Substances

  • DNA, Antisense
  • Histones
  • RNA Precursors
  • RNA, Antisense
  • RNA, Catalytic
  • RNA, Ribosomal
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • RNA
  • DNA