Severe amoebic placentitis in a horse caused by an Acanthamoeba hatchetti isolate identified using next-generation sequencing

Angela P Begg; Kristen Todhunter; Shannon L Donahoe; Mark Krockenberger; Jan Slapeta

doi:10.1128/JCM.01071-14

Severe amoebic placentitis in a horse caused by an Acanthamoeba hatchetti isolate identified using next-generation sequencing

J Clin Microbiol. 2014 Aug;52(8):3101-4. doi: 10.1128/JCM.01071-14. Epub 2014 May 14.

Authors

Angela P Begg¹, Kristen Todhunter¹, Shannon L Donahoe², Mark Krockenberger², Jan Slapeta³

Affiliations

¹ Vetnostics, North Ryde, New South Wales, Australia.
² Faculty of Veterinary Science, University of Sydney, New South Wales, Australia.
³ Faculty of Veterinary Science, University of Sydney, New South Wales, Australia jan.slapeta@sydney.edu.au.

Abstract

A case of amoebic placentitis in a mare from eastern Australia was diagnosed postpartum by histopathological examination of the placenta. The identity of the etiological agent was confirmed as Acanthamoeba hatchetti by use of diversity profiling based on a next-generation sequencing approach.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Acanthamoeba / genetics
Acanthamoeba / isolation & purification*
Amebiasis / diagnosis
Amebiasis / parasitology
Amebiasis / pathology
Amebiasis / veterinary*
Animals
Australia
DNA, Protozoan / chemistry
DNA, Protozoan / genetics
Female
High-Throughput Nucleotide Sequencing
Horse Diseases / diagnosis*
Horse Diseases / parasitology
Horse Diseases / pathology
Horses
Molecular Sequence Data
Placenta Diseases / diagnosis
Placenta Diseases / parasitology
Placenta Diseases / pathology
Placenta Diseases / veterinary*
Pregnancy
Pregnancy Complications, Parasitic / diagnosis
Pregnancy Complications, Parasitic / parasitology
Pregnancy Complications, Parasitic / pathology
Pregnancy Complications, Parasitic / veterinary*

Substances

DNA, Protozoan

Associated data

GENBANK/KJ801938
SRA/SRP041013