Abstract
The Arkansas colorimetric method monitors adherence to isoniazid (INH) by the detection of INH metabolites in urine. Urine samples 4 h after INH administration in 31 human immunodeficiency virus infected children receiving daily or thrice weekly INH preventive therapy were Arkansas test-positive for 29/31 (94%), while acetylisoniazid (AcINH) was detected in 30/31 (97%) using mass spectrometry. At 24, 48 and 72 h, only 78%, 23% and 0 samples, respectively, were Arkansas-positive, while INH or AcINH was detected in respectively 94%, 69% and 33%. The Arkansas test reliably predicted INH ingestion at a clinic visit 4 h after morning doses, but did not perform well at 24 h.
Trial registration:
ClinicalTrials.gov NCT00330304.
Publication types
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Comparative Study
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Randomized Controlled Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Age Factors
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Antitubercular Agents / administration & dosage
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Antitubercular Agents / pharmacokinetics
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Antitubercular Agents / urine*
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Biomarkers / urine
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Biotransformation
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Child
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Chromatography, High Pressure Liquid
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Coinfection*
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Colorimetry
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Drug Monitoring / methods*
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HIV Infections / complications*
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HIV Infections / diagnosis
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Humans
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Isoniazid / administration & dosage
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Isoniazid / analogs & derivatives*
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Isoniazid / pharmacokinetics
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Isoniazid / urine
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Medication Adherence*
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Predictive Value of Tests
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Primary Prevention / methods*
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Reproducibility of Results
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South Africa
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Tandem Mass Spectrometry
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Tuberculosis / complications
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Tuberculosis / diagnosis
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Tuberculosis / prevention & control*
Substances
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Antitubercular Agents
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Biomarkers
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acetylisoniazid
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Isoniazid
Associated data
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ClinicalTrials.gov/NCT00330304